Explainable Machine Learning Reveals 12-Fold Ucp1 Upregulation and Thermogenic Reprogramming in Female Mouse White Adipose Tissue After 37 Days of Microgravity: First AI/ML Analysis of NASA OSD-970

arXiv cs.LG / 4/6/2026

💬 OpinionSignals & Early TrendsIdeas & Deep AnalysisModels & Research

共有:

Key Points

The paper reports an explainable machine learning re-analysis of NASA OSDR dataset OSD-970 (RR-1 mission) using RT-qPCR measurements from 16 female mice after 37 days on the ISS.
It finds a major microgravity-associated thermogenic shift in female white adipose tissue, including a 12.21-fold upregulation of Ucp1 and an overall thermogenesis pathway fold-change of 3.24.
Using multiple ML classifiers with leave-one-out cross-validation, the best model (Random Forest with the top 20 features) achieved strong discrimination between flight and ground samples (AUC 0.922, F1 0.824).
Explainable AI with SHAP highlights Ucp1 as a consistently top predictive feature and identifies Angpt2, Irs2, Jun, and Klf-family transcription factors as key drivers of classification.
PCA shows clear separation between microgravity-exposed and control samples, suggesting rapid thermogenic reprogramming and motivating implications for female astronaut health and metabolic disease research on Earth.

Abstract

Microgravity induces profound metabolic adaptations in mammalian physiology, yet the molecular mechanisms governing thermogenesis in female white adipose tissue (WAT) remain poorly characterized. This paper presents the first machine learning (ML) analysis of NASA Open Science Data Repository (OSDR) dataset OSD-970, derived from the Rodent Research-1 (RR-1) mission. Using RT-qPCR data from 89 adipogenesis and thermogenesis pathway genes in gonadal WAT of 16 female C57BL/6J mice (8 flight, 8 ground control) following 37 days aboard the International Space Station (ISS), we applied differential expression analysis, multiple ML classifiers with Leave-One-Out Cross-Validation (LOO-CV), and Explainable AI via SHapley Additive exPlanations (SHAP). The most striking finding is a dramatic 12.21-fold upregulation of Ucp1 (Delta-Delta-Ct = -3.61, p = 0.0167) in microgravity-exposed WAT, accompanied by significant activation of the thermogenesis pathway (mean pathway fold-change = 3.24). The best-performing model (Random Forest with top-20 features) achieved AUC = 0.922, Accuracy = 0.812, and F1 = 0.824 via LOO-CV. SHAP analysis consistently ranked Ucp1 among the top predictive features, while Angpt2, Irs2, Jun, and Klf-family transcription factors emerged as dominant consensus classifiers. Principal component analysis (PCA) revealed clear separation between flight and ground samples, with PC1 explaining 69.1% of variance. These results suggest rapid thermogenic reprogramming in female WAT as a compensatory response to microgravity. This study demonstrates the power of explainable AI for re-analysis of newly released NASA space biology datasets, with direct implications for female astronaut health on long-duration missions and for Earth-based obesity and metabolic disease research.